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proteome profiler human protease array  (R&D Systems)


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    R&D Systems proteome profiler human protease array
    Proteome Profiler Human Protease Array, supplied by R&D Systems, used in various techniques. Bioz Stars score: 95/100, based on 85 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/human+array/Proteome+Profiler+Human+Protease+Array+Kit/pmc13019076-417-4-10
    Average 95 stars, based on 85 article reviews
    proteome profiler human protease array - by Bioz Stars, 2026-10
    95/100 stars

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    Related Articles

    Phospho-proteomics:

    Article Title: TIM3 + Tumor Associated M2 Macrophages Impair Antitumor T Cell Immunity and Promote Gastric Cancer Progression and Peritoneal Metastasis
    Article Snippet: .. Equal amounts of protein were applied to the Proteome Profiler Human Phospho-Kinase Array Kit (R&D Systems) to assess the phosphorylation status of 22 protein kinases, according to the manufacturer’s instructions. ..

    Imaging:

    Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
    Article Snippet: .. Upon following manufacturer instruction of Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009), high-resolution images were acquired using Chemidoc MP imaging system and processed using Image Lab version 6.0 (Bio-Rad Laboratories, Hercules, CA, USA). .. Quantification of relative protein expressions were done using microarray profile plugin in ImageJ (ImageJ 1.52a, National Institutes of Health, USA).

    Article Title: Adaptable sliding hydrogels enable pericellular pocket formation while enhancing MSC chondrogenesis and survival in 3D
    Article Snippet: .. The kit was the Proteome Profiler Human Protease Array (ARY021B, R&D Systems) and the arrays were imaged using an Invitrogen iBright CL1500 imaging system. ..

    Expressing:

    Article Title: Sequential Invasion Assays Identify Carbonic Anhydrase IX as a Driver of Invasiveness in Epithelial Triple-Negative Breast Cancer cells.
    Article Snippet: 6 number and size of tumorspheres, cultures were imaged by light microscopy at 4x magnification using 204 Visual Dynamix EXI-410 microscope coupled to a Excelis 4k UHD digital camera and CaptaVision+ 205 imaging software. .. 206 Oncoprotein profiling by antibody arrays 207 Oncoprotein profiling was performed by using the Proteome Profiler Human XL Oncology Array kit 208 according to the manufacturer’s instructions (R&D Systems, Inc., Minneapolis, MN, USA, Cat #ARY022B) 209 and cleared whole cell extracts prepared from parental and invasive subpopulations from AnxA6-210 expressing (NSC) and AnxA6-depleted (A6sh) MDA-468 and BT-549 TNBC cells. .. The blots were revealed 211 by enhanced chemiluminescence (ECL) (Perkin Elmer, Waltham, MA), and the intensity of the duplicate 212 spots was quantified by using the particle analysis module in ImageJ software as described recently (40).

    Multiple Displacement Amplification:

    Article Title: Sequential Invasion Assays Identify Carbonic Anhydrase IX as a Driver of Invasiveness in Epithelial Triple-Negative Breast Cancer cells.
    Article Snippet: 6 number and size of tumorspheres, cultures were imaged by light microscopy at 4x magnification using 204 Visual Dynamix EXI-410 microscope coupled to a Excelis 4k UHD digital camera and CaptaVision+ 205 imaging software. .. 206 Oncoprotein profiling by antibody arrays 207 Oncoprotein profiling was performed by using the Proteome Profiler Human XL Oncology Array kit 208 according to the manufacturer’s instructions (R&D Systems, Inc., Minneapolis, MN, USA, Cat #ARY022B) 209 and cleared whole cell extracts prepared from parental and invasive subpopulations from AnxA6-210 expressing (NSC) and AnxA6-depleted (A6sh) MDA-468 and BT-549 TNBC cells. .. The blots were revealed 211 by enhanced chemiluminescence (ECL) (Perkin Elmer, Waltham, MA), and the intensity of the duplicate 212 spots was quantified by using the particle analysis module in ImageJ software as described recently (40).

    Lysis:

    Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
    Article Snippet: .. The adenoid cystic carcinoma cells were added to 100 μL of lysis solution, supplied in Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009) and 10 μL of protease inhibitor (Protease Inhibitor Cocktail I, Tocris, Cat 5500). ..

    Protease Inhibitor:

    Article Title: Functionalized nanoparticle transforms “cold” to “hot” adenoid cystic carcinoma of salivary gland tumour microenvironment in vitro
    Article Snippet: .. The adenoid cystic carcinoma cells were added to 100 μL of lysis solution, supplied in Proteome Profiler Human Apoptosis Array Kit (R&D Systems, Cat RDSARY009) and 10 μL of protease inhibitor (Protease Inhibitor Cocktail I, Tocris, Cat 5500). ..



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    Hyperoxia‐induced senescent fASM secrete higher levels of SASP. Cells cultured till day 7 in normoxic (21% O 2 ) or hyperoxic (50% O 2 ) environment were incubated with fresh growth media in normoxia for 24 h, and the supernatants were collected. Samples were then analyzed for SASP secretion by Eve Technologies Corporation (Calgary, Alberta, Canada) using Luminex xMAP and the Human <t>Cytokine/Chemokine</t> <t>96‐Plex</t> Discovery Assay Array <t>(HD96)</t> panel. (A) Out of the 96 proteins, 80 were detected and visualized using a heatmap. (B) A detailed analysis of each detected marker is shown as fold‐change normalized to control. For statistical analysis, 2‐way ANOVA with a two‐stage linear step‐up procedure of Benjamini, Krieger and Yekutieli test, with individual variances computed for each comparison was applied. False discovery rate < 0.05 was considered significant. Data are presented as box plot of n = 7 cell lines per group.
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    Image Search Results


    Hyperoxia‐induced senescent fASM secrete higher levels of SASP. Cells cultured till day 7 in normoxic (21% O 2 ) or hyperoxic (50% O 2 ) environment were incubated with fresh growth media in normoxia for 24 h, and the supernatants were collected. Samples were then analyzed for SASP secretion by Eve Technologies Corporation (Calgary, Alberta, Canada) using Luminex xMAP and the Human Cytokine/Chemokine 96‐Plex Discovery Assay Array (HD96) panel. (A) Out of the 96 proteins, 80 were detected and visualized using a heatmap. (B) A detailed analysis of each detected marker is shown as fold‐change normalized to control. For statistical analysis, 2‐way ANOVA with a two‐stage linear step‐up procedure of Benjamini, Krieger and Yekutieli test, with individual variances computed for each comparison was applied. False discovery rate < 0.05 was considered significant. Data are presented as box plot of n = 7 cell lines per group.

    Journal: Aging Cell

    Article Title: Targeting Hyperoxia‐Induced Cellular Senescence in Developing Human Airway Cells: Senomorphics Versus Senolytics Versus Antioxidants

    doi: 10.1111/acel.70538

    Figure Lengend Snippet: Hyperoxia‐induced senescent fASM secrete higher levels of SASP. Cells cultured till day 7 in normoxic (21% O 2 ) or hyperoxic (50% O 2 ) environment were incubated with fresh growth media in normoxia for 24 h, and the supernatants were collected. Samples were then analyzed for SASP secretion by Eve Technologies Corporation (Calgary, Alberta, Canada) using Luminex xMAP and the Human Cytokine/Chemokine 96‐Plex Discovery Assay Array (HD96) panel. (A) Out of the 96 proteins, 80 were detected and visualized using a heatmap. (B) A detailed analysis of each detected marker is shown as fold‐change normalized to control. For statistical analysis, 2‐way ANOVA with a two‐stage linear step‐up procedure of Benjamini, Krieger and Yekutieli test, with individual variances computed for each comparison was applied. False discovery rate < 0.05 was considered significant. Data are presented as box plot of n = 7 cell lines per group.

    Article Snippet: Supernatants collected at day 8 from normoxia and hyperoxia‐exposed fASM were analyzed for SASP secretion by Eve Technologies Corporation (Calgary, Alberta, Canada) using the Human Cytokine/Chemokine 96‐Plex Discovery Assay Array (HD96) panel.

    Techniques: Cell Culture, Incubation, Luminex, Marker, Control, Comparison